Home > Term: northern blot
northern blot
1) The separation of sequences or fragments of RNA, partially digested by endonucleases, on an electrophoretic gel.
2) A blot consisting of a sheet of cellulose nitrate or nylon that contains spots of RNA for identification by a suitable molecular probe.
3) Northern blot is a laboratory technique used to detect a specific RNA sequence in a blood or tissue sample. The sample RNA molecules are separated by size using gel electrophoresis. The RNA fragments are transferred out of the gel to the surface of a membrane. The membrane is exposed to a DNA probe labeled with a radioactive or chemical tag. If the probe binds to the membrane, then the complementary RNA sequence is present in the sample.
- Kalbos dalis: noun
- Pramonės šaka / sritis: Health care
- Category: Genetic disorders
- Company: National Library of Medicine
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